Mangesh Jadhav

My general research responsibilities as a postdoctoral senior research associate were leading plant tissue culture, genetic transformation and genome editing team, performing bioinformatics analysis of genomic data and manuscript writing.

In my Ph.D. thesis research, I did genotyping-by-sequencing based high-resolution genetic mapping and discovered consistent genomic regions/genes for productivity and quality traits in peanut. They were further validated in different genetic backgrounds. Also, developed marker-assisted backcross lines with enhanced oleic acid content in the background of five important peanut genotypes. Further, homozygous lines were selected through Kompetitive allele-specific PCR (KASP) assay and background genome recovery was checked through dd-RAD sequencing. Station trial evaluation in large plot led to selection of promising lines, which are under multi-location trial as a part of varietal release.

During my M.Sc. thesis research, I tried to perform Agrobacterium-mediated genetic transformation through both somatic embryogenesis and In-planta method in some genotypes of cotton to check the efficiency of both approaches.

More than the afore-mentioned research experience, during my masters and doctoral studies I was also involved in other collaborative research projects undergoing in respective labs and contributed to the experimentation, preparation of regular progression reports and manuscript writing, resulting in publication in referred journals.